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Buyer documentation guide

How to verify a research peptide COA: a 10-point checklist

Published 2026-10-22 · updated 2026-10-22 · vendor-authored explanatory note

Short answer

A peptide certificate of analysis (COA) is only useful if it describes the exact lot you received. Check that the lot number on the vial matches the report, that a named third-party laboratory issued it, that HPLC purity and LC-MS identity are both reported for that sample, and that the measured quantity matches the labeled amount. If any of those fields is missing, treat the result as unknown and ask the supplier for the lot-specific report.

The 10-point checklist

Work through these in order. The first three items decide whether the document is about your material at all; the rest decide what it actually shows.

  1. Lot match: the batch or lot number printed on the vial label is identical to the lot on the report. A cap color or product name is not a lot ID.
  2. Issuing laboratory: the report names an independent laboratory (not only the seller) and gives enough detail to contact it.
  3. Sample identity: the compound name and labeled quantity on the report match the vial (for example 10 mg, not a different size).
  4. Dates: the sample-received and report dates are present and plausible for the lot you hold.
  5. Purity method: purity is reported with its method, typically reversed-phase HPLC with UV detection, and the value is a number, not a range like 'above 98%' without data.
  6. Chromatogram: a trace or integration table accompanies the purity figure so the main peak and impurities can be seen.
  7. Identity confirmation: mass spectrometry (LC-MS or MALDI) confirms the molecular mass. HPLC purity alone does not prove which molecule is in the vial.
  8. Measured quantity: the report states how much material was found (for example 10.04 mg) so under-filled vials can be spotted.
  9. Additional screens, when claimed: endotoxin (LAL), sterility, or heavy-metal results only count if the report shows the method and result for that lot.
  10. Traceability: the supplier can show the same report on request or through a lookup, and the image has not been cropped to remove the lot or lab fields.

Red flags

Be cautious when the same COA image is reused across several sizes or products, when the lot field is blank or replaced with a color, when a purity figure is shown without any method, when the laboratory name cannot be found, or when the only document offered is a generic 'specification sheet' rather than a test result.

A high HPLC number without mass-spec identity data is incomplete. A report for a different quantity (a 5 mg test offered for a 10 mg vial) does not describe your vial.

How to check a Vitality Chems lot

Enter the lot number printed on your vial at vitalitychems.com/verify. If it matches a published report, the page shows the laboratory, report date, method, reported purity, measured quantity, and a link to the full certificate image. If it does not match, the page offers a request form for the exact lot report instead of substituting another lot.

Reports that identify a sample only by cap color are labeled as such on the testing page, because a color is not a unique, traceable lot identifier.

What a COA cannot tell you

A certificate describes the tested sample on the test date. It does not establish suitability for any use outside qualified laboratory research, stability after shipping or reconstitution, or any biological effect. Keep the report with your lab records alongside the lot number, storage conditions, and receipt date.

Frequently asked

What should a peptide certificate of analysis include?

At minimum: compound name, labeled quantity, lot or batch number, issuing laboratory, sample and report dates, the purity method and result (usually HPLC-UV with a chromatogram), identity confirmation by mass spectrometry, and the measured quantity.

Is HPLC purity enough to confirm a peptide's identity?

No. HPLC purity measures how much of the detected signal belongs to the main peak. Mass spectrometry is needed to confirm that the main peak has the expected molecular mass.

Why does the lot number matter so much?

A COA is evidence only for the batch that was tested. Without a matching lot number there is no link between the document and the vial in front of you.

Can I look up a Vitality Chems lot online?

Yes. Enter the lot number from the vial at vitalitychems.com/verify. Unpublished lots can be requested from the same page.

References

  1. ICH Q2(R2), Validation of Analytical ProceduresSpecificity, accuracy, and reportable range concepts used when reading identity and purity results.
  2. ICH Q14, Analytical Procedure DevelopmentHow analytical procedures and their parameters should be documented.