Buyer documentation guide
How to read a BPC-157 / TB-500 blend certificate of analysis
Published 2026-10-29 · updated 2026-10-29 · vendor-authored explanatory note
Short answer
A blend certificate has to answer the single-compound questions twice. For a BPC-157 / TB-500 vial that means two resolved HPLC peaks, a measured quantity for each peptide (not one combined figure), and identity evidence for each: about m/z 1419.7 / 710.4 for BPC-157 and 889.5 / 445.3 for TB-500. A single purity number for the whole vial cannot show that both components are present in the stated ratio.
Catalog materials discussed on this page
| Item | What to look for | Why it matters |
|---|---|---|
| Lot or batch | The lot printed on the vial label | Ties the report to the vial in hand rather than a sister batch. |
| Chromatogram | Two main peaks, each labeled and resolved from the other | Overlapping or unlabeled peaks make per-component purity unreadable. |
| Quantity per component | Separate mg values for BPC-157 and TB-500 | Shows the stated ratio (for example 10 mg / 10 mg) was met for both. |
| Purity | Area percent for each component, or for the sum, stated clearly | A combined figure hides which peptide carries the impurities. |
| Identity | Observed m/z for BPC-157 (about 1419.7 [M+H]+, 710.4 [M+2H]2+) and TB-500 (about 889.5, 445.3) | HPLC alone does not identify a peak. |
| Laboratory and date | Named lab, report date, and a way to confirm the report with that lab | Lets a reader check the document was issued as shown. |
Why blends are harder to document
A single-compound vial needs one purity result, one identity result and one quantity. A blend needs each of those per component, plus evidence that the components do not interfere with each other's measurement. BPC-157 (1419.5 g/mol) and TB-500 (889.0 g/mol) are different enough in size and polarity that a reversed-phase gradient normally separates them, so a competent report can show both peaks on one chromatogram.
The same logic applies to larger multi-peptide vials, such as blends that add GHK-Cu or KPV. Each additional component adds another peak to label, another quantity to report, and another identity to confirm.
Reading the quantities
Quantity is measured against a reference standard for each peptide and reported in mg per vial. Measured values a little above the label figure are common because manufacturers fill with a margin; a value well below the label for one component and not the other suggests a ratio problem. Note that the measured mass of peptide is not the same as the weight of powder in the vial, which also contains counter-ions and residual water.
As a worked example, the published Vitality Chems BPC-157 / TB-500 10 mg / 10 mg report (Vanguard Laboratory, reported 2026-02-26, HPLC-UV/VIS) lists BPC-157 at 11.26 mg and TB-500 at 12.10 mg, with purity above 99.80%. That report is an HPLC result; it identifies the sample by cap color rather than a printed lot number and does not include a mass spectrum, so identity for this lot is not documented by LC-MS. We state that limitation here so readers can weigh the document accurately, and the original PDF is available on request.
Confirming a report with the issuing lab
Several testing laboratories run public lookups. Freedom Diagnostics Testing offers a certificate lookup by accession number, search code or company name that returns the lab's own PDF. Janoshik Analytical verifies reports by task number and unique key on its verification page. Where a lookup exists, compare the lab's copy with the vendor's copy line by line: sample name, lot, dates and every result.
Where no lookup exists, ask the vendor for the original PDF and the lab's contact for the report, and check that the lab name, address and accreditation claims match the lab's own website.
- Same lot on the report and the vial.
- Two labeled peaks and two quantities.
- Mass data for each component, or a clear statement that identity was not tested by MS.
- Lab copy matches vendor copy where a lookup exists.
Related on Vitality Chems
- BPC-157 / TB-500 blend — 10 mg / 10 mg vial and its published report.
- BPC-157 — single-compound vial.
- TB-500 — single-compound vial with LC-MS identity.
- KLOW multi-peptide blend — four-component vial.
- Peptide COA checklist — the single-compound version.
- BPC-157 vs TB-500 compared — identity data side by side.
Frequently asked
What should a BPC-157 / TB-500 blend COA show?
Two labeled, resolved HPLC peaks; a measured mg quantity for each peptide; purity per component or clearly stated for the sum; identity data for each peptide; and the lot number, lab name and report date.
Is one purity number enough for a blend?
No. A single figure for the whole vial cannot show that both peptides are present in the stated ratio or which one carries the impurities.
What m/z values identify BPC-157 and TB-500 in a blend?
BPC-157: about m/z 1419.7 [M+H]+ and 710.4 [M+2H]2+. TB-500: about m/z 889.5 [M+H]+ and 445.3 [M+2H]2+ (monoisotopic).
How do I check a blend COA is genuine?
Use the issuing lab's lookup where one exists (for example Freedom Diagnostics by accession number or Janoshik by task number and key) and compare the lab's copy with the vendor's copy. Otherwise request the original PDF and confirm the lab's details on its own website.
Are BPC-157 / TB-500 blends approved drugs?
No. They are supplied for laboratory research use only, are not approved drugs, and are not for human or animal use.
References
- Freedom Diagnostics Testing: Certificate of Analysis LookupPublic lookup by search code, accession number or company name (checked 2026-10-29).
- Janoshik Analytical: VerificationReport verification by task number and key (checked 2026-10-29).
- Lian Z, et al. Characterization of synthetic peptide therapeutics using liquid chromatography-mass spectrometry: challenges, solutions, pitfalls. J Am Soc Mass Spectrom. 2021Identity and impurity assessment of synthetic peptides by LC-MS.
- PubChem: BPC-157 (CID 9941957)Monoisotopic mass 1418.704.
- PubChem: TB-500 (CID 62707662)Monoisotopic mass 888.492.
